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on the list of issues in the development or technology of SMC from ASCs is always to enhance microenvironments for successful linear-distinct differentiation, cell proliferation, and routine maintenance of contractile phenotype. Oxygen is a crucial element from the ideal microenvironment [9]. Even though most mobile cultures and expansions are executed in vitro at 20% oxygen focus, the actual oxygen tensions of numerous tissues in physiological atmosphere are much decreased: oxygen concentration in adipose tissue is around 2–eight% [ten].
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Expression of SMC-precise genes and proteins, single mobile contraction assay, and collagen gel lattice assay all reveal five% oxygen given that the optimal oxygen environment during differentiation.
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